Difference between revisions of "Part:BBa K2587011"
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This construct contains a constitutive promoter (J23102),an RBS (B0032), a fluorescent protein: GFP (E0040m) and a terminator (B0015).  
 
This construct contains a constitutive promoter (J23102),an RBS (B0032), a fluorescent protein: GFP (E0040m) and a terminator (B0015).  
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It is built using the CIDAR MoClo toolbox for Golden Gate cloning for E.coli and can be used to track the cells due to constitutive expression of a reporter.
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It is built using the CIDAR MoClo toolbox for Golden Gate cloning for <i>E.coli</i> and can be used to track the cells due to constitutive expression of a reporter.
  
 
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Revision as of 13:41, 16 October 2018


P_RBS_gfp_T

This construct contains a constitutive promoter (J23102),an RBS (B0032), a fluorescent protein: GFP (E0040m) and a terminator (B0015). It is built using the CIDAR MoClo toolbox for Golden Gate cloning for E.coli and can be used to track the cells due to constitutive expression of a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706