Difference between revisions of "Part:BBa K2817000"

 
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<partinfo>BBa_K2817000 short</partinfo>
 
<partinfo>BBa_K2817000 short</partinfo>
  
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When nitric oxide is present in the environment, the transcription factor NorR will be activated to bind the promoter PnorV to initiate the expression of the blue chromoprotein.
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There are some natural nitric oxide sensors in E. coli, in which the enhancer binding protein NorR is specific and can only react with nitric oxide and cannot interact with other nitrogen forms. NorR binds to three conserved sites on the NorV promoter (PnorV) via the C terminal HTH domain. When nitric oxide is absent, the N-terminal domain GAF of NorR blocks the central domain AAA+ to inhibit its binding to the transcription factor σ 54, and thereby prevent transcription of the nitric oxide reductase NorV. When nitric oxide binds to the GAF domain of NorR, it will release the AAA+ domain, allowing σ 54 to initiate transcription of NorV. Therefore, when nitric oxide is present in the environment, the promoter PnorV will initiate the expression of the blue chromoprotein.
  
 
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Revision as of 13:08, 16 October 2018


PnorV-RBS-amilCP

There are some natural nitric oxide sensors in E. coli, in which the enhancer binding protein NorR is specific and can only react with nitric oxide and cannot interact with other nitrogen forms. NorR binds to three conserved sites on the NorV promoter (PnorV) via the C terminal HTH domain. When nitric oxide is absent, the N-terminal domain GAF of NorR blocks the central domain AAA+ to inhibit its binding to the transcription factor σ 54, and thereby prevent transcription of the nitric oxide reductase NorV. When nitric oxide binds to the GAF domain of NorR, it will release the AAA+ domain, allowing σ 54 to initiate transcription of NorV. Therefore, when nitric oxide is present in the environment, the promoter PnorV will initiate the expression of the blue chromoprotein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]