Difference between revisions of "Part:BBa K2686001:Experience"
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | The part was designed and constructed in a pET14 vector. The sequence was confirmed using sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32. | + | The part was designed and constructed in a pET14 vector. The sequence was confirmed using sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.16calckDa for the monomer and 1.93MDa for the 60-mer). DLS measurements also show that our protein cage self-assembles into the 60-mer. |
===Applications of BBa_K2686001=== | ===Applications of BBa_K2686001=== | ||
Revision as of 12:57, 16 October 2018
The part was designed and constructed in a pET14 vector. The sequence was confirmed using sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.16calckDa for the monomer and 1.93MDa for the 60-mer). DLS measurements also show that our protein cage self-assembles into the 60-mer.
Applications of BBa_K2686001
User Reviews
UNIQ0b20767c65ee7081-partinfo-00000000-QINU UNIQ0b20767c65ee7081-partinfo-00000001-QINU