Difference between revisions of "Part:BBa K2807012"

 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2807012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2807012 SequenceAndFeatures</partinfo>
  

Revision as of 12:21, 16 October 2018


eGFP-T2A-mCherry

Inspired by the work of Martin et al. (2018), as well as WPI Worcester 2016 iGEM project, we designed a eGFP-T2A-mCherry dual fluorescence reporter plasmid system. This plasmid consists of two fluorescence markers, an EGFP gene and a mCherry gene. Here, mCherry is expressed constitutively and used as a marker for transfection efficiency and expression levels of a base editing fusion protein, dCAS-XTEN-APOBEC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]