Difference between revisions of "Part:BBa K2812000:Design"

(Source)
(Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
Genomic sequence from M. primoryensis. Protein sequence obtained from Guo et al. 2017 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550230/)
+
Genomic sequence from M. primoryensis. Protein sequence obtained from Guo et al. 2017.  
 
+
 
Construct synthesized ''de novo'' by IDT.
 
Construct synthesized ''de novo'' by IDT.
  
 
===References===
 
===References===
 
Guo, S., Stevens, C., Vance, T., Olijve, L., Graham, L., Campbell, R., . . . Davies, P. (2017). Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Science Advances, 1-10.
 
Guo, S., Stevens, C., Vance, T., Olijve, L., Graham, L., Campbell, R., . . . Davies, P. (2017). Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Science Advances, 1-10.

Revision as of 09:39, 16 October 2018


Carbohydrate-binding domain from MpIBP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence codon optimized for E. coli.


Source

Genomic sequence from M. primoryensis. Protein sequence obtained from Guo et al. 2017. Construct synthesized de novo by IDT.

References

Guo, S., Stevens, C., Vance, T., Olijve, L., Graham, L., Campbell, R., . . . Davies, P. (2017). Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Science Advances, 1-10.