Difference between revisions of "Part:BBa K2570006"

Latest revision as of 08:45, 16 October 2018

atoD

This sequence encodes for atoD, which is the first enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with BBa_K2570007 , it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with BBa_K2570007 and BBa_K2570008, aiming to eliminate unpleasant axilla odors E-3M2H by converting acids into alcohols.

Fig.1 The structure of atoD.

The atoD sequence was obtained from E.coli BW25113 by PCR

Fig.1 PCR result of atoD.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This sequence encodes for atoD, which is the first enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570007 BBa_K2570007]
 , it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570007 BBa_K2570007] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570008 BBa_K2570008], aiming to eliminate unpleasant axilla odors E-3M2H by converting acids into alcohols.
-[[File:T--FJNU-China--Basic_part-atoD_1.jpg|600px|thumb|center|Fig.The structure of atoD.]]
+[[File:T--FJNU-China--Basic_part-atoD_1.jpg|600px|thumb|center|Fig.1 The structure of atoD.]]
+The atoD sequence was obtained from ''E.coli BW25113'' by PCR
+[[File:T--FJNU-China--Basic_part-atoD_2.jpg|600px|thumb|center|Fig.1 PCR result of atoD.]]
 <!-- Add more about the biology of this part here