Difference between revisions of "Part:BBa K2570006:Design"

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===Design Notes===
 
===Design Notes===
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The atoD sequence was obtained from E.coli BW25113 by PCR
+
 
 +
Primers:
 +
Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’
 +
 
 +
Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’
 +
 
  
 
===Source===
 
===Source===
  
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1. Organism="Escherichia coli str. K-12 substr. MG1655". Protein_id="AAC75281.1". product="acetyl-CoA:acetoacetyl-CoA transferase subunit alpha".  
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Organism="''Escherichia coli str. K-12 substr. MG1655''". Protein_id="AAC75281.1". product="acetyl-CoA:acetoacetyl-CoA transferase subunit alpha".  
  
 
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2323447&to=2324109
 
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2323447&to=2324109

Latest revision as of 08:44, 16 October 2018


atoD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’

Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’


Source

Organism="Escherichia coli str. K-12 substr. MG1655". Protein_id="AAC75281.1". product="acetyl-CoA:acetoacetyl-CoA transferase subunit alpha".

https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2323447&to=2324109

References

Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.