Difference between revisions of "Part:BBa P10101:Experience"
 
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===Applications of BBa_P10101===
 
===Applications of BBa_P10101===
  
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The Cardiff iGEM team of 2018 characterised this promoter using the [https://parts.igem.org/Part:BBa_K2810001 GUS] and [https://parts.igem.org/Part:BBa_K2810009 mCherry] reporter genes. We compared the expression of this promoter to that of the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter] using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
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The [http://2018.igem.org/Team:Cardiff_Wales 2018 Cardiff iGEM team] characterised this promoter using the [https://parts.igem.org/Part:BBa_K2810001 GUS] and [https://parts.igem.org/Part:BBa_K2810009 mCherry] reporter genes. We compared the expression of this promoter to that of the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter] using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
  
  
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[[File:35S%2BRTBV_GUS_NosT_results.png|700px|thumb|center|Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.]]
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[[File:BBa_K2810002_OR_BBa_P10101_AND_K2810001_results.png|700px|thumb|center|Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.]]
  
  
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[[File:35S%2BRTBV_mCherry_NosT_results.png|700px|thumb|center|Figure 2)  
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[[File:BBa_K2810002_OR_BBa_P10101_AND_K2810009_results.png|700px|thumb|center|Figure 2)  
 
The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too, suggesting that the 35S CaMV promoter has expression 1000x that of the background level, and between 10-100x that of RTBV.]]
 
The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too, suggesting that the 35S CaMV promoter has expression 1000x that of the background level, and between 10-100x that of RTBV.]]
  

Latest revision as of 08:44, 16 October 2018


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Applications of BBa_P10101

The [http://2018.igem.org/Team:Cardiff_Wales 2018 Cardiff iGEM team] characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.



Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.




Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too, suggesting that the 35S CaMV promoter has expression 1000x that of the background level, and between 10-100x that of RTBV.





























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