Difference between revisions of "Part:BBa K2656101"
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<partinfo>BBa_K2656101 short</partinfo> | <partinfo>BBa_K2656101 short</partinfo> | ||
Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease. | Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease. | ||
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+ | This composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com '''BioArt DNA toolkit palette: Printone '''], as it can be used to express a green bright color with medium intensity. | ||
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This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]: | This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]: |
Latest revision as of 08:31, 16 October 2018
sfGFP transcriptional unit 2
Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.
This composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green bright color with medium intensity.
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
- BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
- BBa_K2656013: the BBa_I746916 sfGFP sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
This transcriptional unit has been used to characterize the RBS basic part BBa_K2656009. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and the results of the next graphs have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
Table 1. Optimized parameters for the BBa_ K2656101. | |||
Parameter | Value | ||
Translation rate p | p = 0.2059 min-1 | ||
Dilution rate μ | μ = 0.01492 min-1 | ||
PoI degradation rate | RBS Experiment 2: dp = 0.00818 min-1 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 78