Difference between revisions of "Part:BBa K2596005:Design"

 
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===Design Notes===
 
===Design Notes===
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We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through another silent mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.  
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We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.
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===Source===
 
===Source===

Revision as of 04:49, 16 October 2018


cscB (sucrose permease) from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.

Source

E. coli

References