Difference between revisions of "Part:BBa K2596004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment | + | PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment. |
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<partinfo>BBa_K2596004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2596004 SequenceAndFeatures</partinfo> | ||
| − | Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region | + | Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region. We did not include downstream base pairs due to ambiguity in the true start codon. |
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Revision as of 04:39, 16 October 2018
PidiA from Synechococcus elongatus PCC 7942
This is a ferrous-ion repressible promoter for cyanobacteria Synechococcus elongatus PCC 7942.
Usage and Biology
PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region. We did not include downstream base pairs due to ambiguity in the true start codon.