Difference between revisions of "Part:BBa K2599005"
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| + | 1. Iwatani, S., et al. (2007). "Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14." Biosci Biotechnol Biochem 71(8): 1984-1992. | ||
Revision as of 04:19, 16 October 2018
Antimicrobial peptide - Lacticin Z
This biobrick is the basic part of the Lacticin Z, more information please look for the composite part (BBa_K2599013).
Figure 1. Basic part of Lacticin Z
Introduction
Lacticin Z, produced by Lactoccus lactis QU 14, has no leader sequence or signal peptide. Also, it exhibits a nanomolar range of MICs against various gram-positve bacteria. Consequently, Lacticin Z indeed shows the best antibacterial activity in our experiment.
Mechanism of Lacticin Z
The bacteriocins inhibit their target organisms through pore formation. Though the mechanism of each inhibition is vary from species to species, the general process is conserved. To see more details, please search for our project page.
Features of Lacticin Z
1. Species Specific
Bacteriocins are antimicrobial peptides that will kill or inhibit bcterial strains closely related or non-related to produced bacteria, but will not harm the bacteria themselves by specific immunity proteins. The organisims that Lacticin Z targets including Enterococcus faecium, Bacillus subtilis, Bacillus coagulans, etc. More target organisms can be found on [http://bactibase.hammamilab.org/BAC170 bactibase].
2. Eco-friendly
Since Lacticin Z is a polypeptide naturally produced by bacteria itself and can inhibit other bacteria without much environment impact. It don't pose threat to other organisms like farm animals or humans. Therefore, this toxin will not cause safety problem.
3. Biodegradable
Lacticin Z is a short peptide that will degrade in a short time. After degradation, this antibacterial peptide is harmless to our environment.
Experiment Result
Cloning
We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Lacticin Z sequence length is around 153 b.p.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
1. Iwatani, S., et al. (2007). "Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14." Biosci Biotechnol Biochem 71(8): 1984-1992.