Difference between revisions of "Part:BBa K2596003"
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===Usage and Biology===
 
===Usage and Biology===
  
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PpsbA2 is a light-inducible promoter native to cyanobacteria [6]. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism [7]. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light [8].  
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PpsbA2 is a light-inducible promoter native to cyanobacteria. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light.  
  
 
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<partinfo>BBa_K2596003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2596003 SequenceAndFeatures</partinfo>
  
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For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length. The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins [9].
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The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins. For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length.
  
 
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Revision as of 04:17, 16 October 2018


PpsbA2 from Synechococcus elongatus PCC 7942

This a high-light inducible promoter from cyanobacteria Synechococcus elongatus PCC 7942

Usage and Biology

PpsbA2 is a light-inducible promoter native to cyanobacteria. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins. For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length.