Difference between revisions of "Part:BBa K2796028"
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Here is the final device that we designed for this project. | Here is the final device that we designed for this project. | ||
[[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]] | [[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]] | ||
| − | More details you can | + | It combines with exosome booster and miR attacker under control of induced promoter.More details and description you can obtain in our wiki! |
| + | [[File:T--LZU-CHINA--design1.png|600px|thumb|center|]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:10, 16 October 2018
Exosome booster
- Exosome booster refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified STEAP3 (involved in exosomebiogenesis), syndecan-4(SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 192
Illegal BglII site found at 1777 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2657
Illegal AgeI site found at 3277 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1367
Here is the final device that we designed for this project.
It combines with exosome booster and miR attacker under control of induced promoter.More details and description you can obtain in our wiki!
Sequence and Features
Assembly Compatibility: