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| − | == Punch tool cleaning protocol ==
| + | [[ Punch tool cleaning protocol]] |
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| − | Pull the punch tool apart.
| + | [[TE Buffer for thesis paper punches]] |
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| − | Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol. Ethanol is preferred as it dries much faster. Strong detergents should also be avoided.
| + | [[E. coli transformation]] |
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| − | Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool, so it is best to blot until all liquid is gone. It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.
| + | [[Reading Gel Images]] |
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| − | == TE Buffer for thesis paper punches==
| + | [[How to check a part]] |
| − | 100 mmol Tris
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| − | 5.1 mmol EDTA
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| − | can store paper either open or folded
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Latest revision as of 20:26, 9 July 2008
Punch tool cleaning protocol
TE Buffer for thesis paper punches
E. coli transformation
Reading Gel Images
How to check a part
