Difference between revisions of "Part:BBa K2610006"

Latest revision as of 21:54, 15 October 2018

pMalK

The gene MalK encodes for the maltose/maltodextrin import ATP-binding protein MalK, allowing for active transport of maltose across the cell membrane of E.coli. (Maurer et al, 2004)

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the MalK promoter is downregulated by a variety of antibiotics.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2610006 short</partinfo>
-The gene MalK encodes for the maltose/maltodextrin import ATP-binding protein MalK, allowing for active transport of maltose across the cell membrane of E.coli.
+The gene MalK encodes for the maltose/maltodextrin import ATP-binding protein MalK, allowing for active transport of maltose across the cell membrane of E.coli. <span class="plainlinks">[https://jb.asm.org/content/187/1/304 (Maurer et al, 2004)]</span>
+===Usage and Biology===
+<p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br>
+[[File:T--Leiden--heatmap.png|750px]] <br>
+<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>
+As can be observed in Figure 1 the MalK promoter is downregulated by a variety of antibiotics.
 <!-- Add more about the biology of this part here