Difference between revisions of "Part:BBa K2610004"
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<partinfo>BBa_K2610004 short</partinfo>
 
<partinfo>BBa_K2610004 short</partinfo>
  
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The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR. This enzyme is specifically upregulated by oxidative agents, as demonstrated by previous research. It has been shown that the promoter of KatG fused to the lux operon can be strongly induced by hydrogen peroxide in a dose-dependent response. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25384482/ (Zhang et al, 2015]</span><span class="plainlinks">[https://link.springer.com/article/10.1007%2Fs00253-003-1418-0 (Mitchell et al, 2004]</span>
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The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR. This enzyme is specifically upregulated by oxidative agents, as demonstrated by previous research. It has been shown that the promoter of KatG fused to the lux operon can be strongly induced by hydrogen peroxide in a dose-dependent response. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25384482/ (Zhang et al, 2015)]</span><span class="plainlinks">[https://link.springer.com/article/10.1007%2Fs00253-003-1418-0 (Mitchell et al, 2004)]</span>
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Revision as of 21:35, 15 October 2018


pKatG

The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR. This enzyme is specifically upregulated by oxidative agents, as demonstrated by previous research. It has been shown that the promoter of KatG fused to the lux operon can be strongly induced by hydrogen peroxide in a dose-dependent response. (Zhang et al, 2015)(Mitchell et al, 2004)


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the KatG promoter is activated by hydrogen peroxide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]