Difference between revisions of "Part:BBa K2719000:Design"

 
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===Design Notes===
 
===Design Notes===
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Optimized for E.coli BL21
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Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to other proteins and for this is necessary to use a polyprolyne linker to maintain the original three dimensional structure of the protein.This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013).
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===Source===
 
===Source===

Revision as of 21:12, 15 October 2018


GST


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to other proteins and for this is necessary to use a polyprolyne linker to maintain the original three dimensional structure of the protein.This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013).

Source

Purification tag.

References