Difference between revisions of "Part:BBa K2675013"

 
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<partinfo>BBa_K2675013 short</partinfo>
 
<partinfo>BBa_K2675013 short</partinfo>
  
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Synthetic RBS designed for PelB-SAIRGA
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This part is a synthetic RBS designed for PelB-SAIRGA ([[Part:BBa_K2675003|BBa_K2675003]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2].
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This RBS was used to drive the expression of PelB-SAIRGA ([[Part:BBa_K2675003|BBa_K2675003]]) under the control of the [[Part:BBa_J23100|BBa_J23100]] promoter in the composite part [[Part:BBa_K2675043|BBa_K2675043]].
  
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Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
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Translation Initiation Rate (au) : 413347,83
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dG_total (kcal/mol) : -12,92
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dG_mRNA_rRNA (kcal/mol) : -22,03
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dG_spacing (kcal/mol) : 0
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dG_standby (kcal/mol) : 0
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dG_start (kcal/mol) : -2,76
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dG_mRNA (kcal/mol) : -12,44
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Accuracy (warnings) : NoEQ
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<h2 style="font-weight:800;">REFERENCES</h2>
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<p style="font-size:15px;" class="bibliographie">[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.</p>
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<p style="font-size:15px;" class="bibliographie">[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.</p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 21:04, 15 October 2018


Synthetic RBS designed for PelB-SAIRGA

This part is a synthetic RBS designed for PelB-SAIRGA (BBa_K2675003) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of PelB-SAIRGA (BBa_K2675003) under the control of the BBa_J23100 promoter in the composite part BBa_K2675043. 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	413347,83
dG_total (kcal/mol)	:	-12,92
dG_mRNA_rRNA (kcal/mol)	:	-22,03
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-12,44
Accuracy (warnings)	:	NoEQ


REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]