Difference between revisions of "Part:BBa K2570006:Design"
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===Design Notes=== | ===Design Notes=== | ||
The atoD sequence was obtained from ''E.coli BW25113'' by PCR | The atoD sequence was obtained from ''E.coli BW25113'' by PCR | ||
+ | |||
+ | Primers: | ||
+ | Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’ | ||
+ | Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’ | ||
+ | |||
+ | [[File:T--FJNU-China--Basic_part-atoD_2.jpg|600px|thumb|center|Fig.1 PCR result of atoD.]] | ||
===Source=== | ===Source=== |
Revision as of 20:00, 15 October 2018
atoD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The atoD sequence was obtained from E.coli BW25113 by PCR
Primers: Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’ Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’
Source
Organism="Escherichia coli str. K-12 substr. MG1655". Protein_id="AAC75281.1". product="acetyl-CoA:acetoacetyl-CoA transferase subunit alpha".
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2323447&to=2324109
References
Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.