Difference between revisions of "Part:BBa K2570006:Design"
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===Design Notes===
 
===Design Notes===
 
The atoD sequence was obtained from ''E.coli BW25113'' by PCR
 
The atoD sequence was obtained from ''E.coli BW25113'' by PCR
 +
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Primers:
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Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’
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Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’
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 +
[[File:T--FJNU-China--Basic_part-atoD_2.jpg|600px|thumb|center|Fig.1 PCR result of atoD.]]
  
 
===Source===
 
===Source===

Revision as of 20:00, 15 October 2018


atoD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The atoD sequence was obtained from E.coli BW25113 by PCR

Primers: Fwd: 5’-tttgggctaacaggaggaattaaccatgaaaacaaaattgatgacattac-3’ Rev: 5’-gcatccattatatctccttctcgagttatttgctctcctgtgaaac-3’

Fig.1 PCR result of atoD.

Source

Organism="Escherichia coli str. K-12 substr. MG1655". Protein_id="AAC75281.1". product="acetyl-CoA:acetoacetyl-CoA transferase subunit alpha".

https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2323447&to=2324109

References

Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.