Difference between revisions of "Part:BBa K2644004"
 
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<partinfo>BBa_K2644004 short</partinfo>
 
<partinfo>BBa_K2644004 short</partinfo>
  
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An operator to control the expression of fluorescent protein by binding As3+
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An operator to control the expression of smURFP by binding ArsR protein.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2644004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2644004 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2644004 parameters</partinfo>
 
<partinfo>BBa_K2644004 parameters</partinfo>
 
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===Usage===
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ArsR operator is consist of an ArsR promoter and binding site which the ArsR repressor protein binds to. When combined with ArsR, the gene behind can not be transcribed. Conversely, when the ArsR dissociates from the promoter in the presence of arsenite, the gene behind can work as normal.
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===Biology===
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In order to control the expression of target gene by changing the concentration of As(III), we design a operator composing of ArsR promoter and ArsR repressor protein binding site.
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===Reference===
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[1]Davide Merulla1, Nina Buffi2, Siham Beggah1, Fre ́ de ́ ric Truffer3, Martial Geiser3, Philippe Renaud2 and Jan Roelof van der Meer1. Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem. [J] Current Opinion in Biotechnology 2013, 24:534–541

Latest revision as of 18:01, 15 October 2018


ArsR operator

An operator to control the expression of smURFP by binding ArsR protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

ArsR operator is consist of an ArsR promoter and binding site which the ArsR repressor protein binds to. When combined with ArsR, the gene behind can not be transcribed. Conversely, when the ArsR dissociates from the promoter in the presence of arsenite, the gene behind can work as normal.

Biology

In order to control the expression of target gene by changing the concentration of As(III), we design a operator composing of ArsR promoter and ArsR repressor protein binding site.

Reference

[1]Davide Merulla1, Nina Buffi2, Siham Beggah1, Fre ́ de ́ ric Truffer3, Martial Geiser3, Philippe Renaud2 and Jan Roelof van der Meer1. Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem. [J] Current Opinion in Biotechnology 2013, 24:534–541