Difference between revisions of "Part:BBa K2559001"

Latest revision as of 17:49, 15 October 2018

Bacterial cellulose synthase H (BcsH)

The BBa_K2559002 is coding a cellulose complement protein A (CCPA) which is also regarded as Bacterial cellulose synthase H (BCSH).

Usage and Biology

BCSH is firstly identified in the Komagataeibacter andGluconacetobacter and is required for the activity of Bacterial cellulose synthase (BCS) in K. xylinus and Komagataeibacter hansenii.It has been shown to interact with BCSD, and participates in assisting the arrangement of glucan chains into crystalline ribbons in periplasm. The functions of BCSH accounts for the extremely high activity of BCS in K. xylinus.

Figure 1 The enzyme digestion of the biobrick with EcoRI and PstI

We introduced bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria.

Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement

Measurement of cyanobacteria glucose (3 repeats). The same of amount of transgenic cyanobacteria with bcsZH-ABCD-bglX genes and wild-type were treated with lysozyme to break the cells. Due to lacking a direct way to measure the content of cellu;ose in bacterial cell wall. Therefore, glucose can be used as an alternative parameter for measuring the content of cellulose since it can be digested into glucose by cellulose. The differences between the red column and blue column indicated that the content of bacteria cellulose.

Figure 2 The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group and control group.

The part is facilitate the in-depth research for other teams!

Reference :

1.Romling U & Galperin MY (Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. (Translated from eng) Trends Microbiol 23(9):545-557 (in eng).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 746
Illegal AgeI site found at 907
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 351

@@ Line 1: / Line 1: @@
 <partinfo>BBa_K2559001 short</partinfo>
-The BBa_K2559001 is coding a cellulose complement protein A (ccpA) .
+The BBa_K2559002 is coding a cellulose complement protein A (CCPA) which is also regarded as Bacterial cellulose synthase H (BCSH).
 ===Usage and Biology===
-It is encoded exclu-sively in the Komagataeibacter/Gluconacetobacter lineage and is required for the BCS activity in K. xylinus and Komagataeibacter hansenii  It has been shown to interact with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons. in the periplasm. This unique organization might account for the extremely high activity of the K. xylinus BCS.
+BCSH is firstly identified in the Komagataeibacter andGluconacetobacter and is required for the activity of Bacterial cellulose synthase (BCS) in K. xylinus and Komagataeibacter hansenii.It has been shown to interact with BCSD, and participates in assisting the arrangement of glucan chains into crystalline ribbons in periplasm. The functions of BCSH accounts for the extremely high activity of BCS in K. xylinus.
-[[File:Scau-china-2018-6.png|800px|thumb|center|The double digestion of biobrick with EcoRI and PstI]]
+[[File:Scau-china-2018-6.png|800px|thumb|center|Figure 1 The enzyme digestion of the biobrick with EcoRI and PstI]]
 We introduced bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria.
-Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement
+===Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement===
-Cyanobacteria glucose content measurement (3 repeat). The same of quality of transferred cyanobacteria with bcsZH-ABCD-bglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable.
+Measurement of cyanobacteria glucose (3 repeats). The same of amount of transgenic cyanobacteria with bcsZH-ABCD-bglX genes and wild-type were treated with lysozyme to break the cells. Due to lacking a direct way to measure the content of cellu;ose in bacterial cell wall. Therefore, glucose can be used as an alternative parameter for measuring the content of cellulose since it can be digested into glucose by cellulose.
-[[File:Scau-china-2018-4.png|800px|thumb|center|	The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group  and control group. ]]
+The differences between the red column and blue column indicated that the content of bacteria cellulose.
+[[File:Scau-china-2018-4.png|800px|thumb|center|Figure 2 The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group  and control group. ]]
 The part is facilitate the in-depth research for other teams!
 Reference :
 .Romling U & Galperin MY (Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. (Translated from eng) Trends Microbiol 23(9):545-557 (in eng).