Difference between revisions of "Part:BBa K2599016"
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===Improvement part===
 
===Improvement part===
  
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The GS linker we used in this improvement part was also provided by NCTU_Formosa 2016. It contained 18 amino acid sequence.
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The GS linker we used in this improvement part was also provided by NCTU_Formosa 2016. It contained 18 amino acid sequence, which is a Gly-Gly-Ser repeated linker.
  
 
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Revision as of 16:32, 15 October 2018


T7 Promoter+RBS+Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag

NCTU_Formosa 2018 designed this sequence to improve the part from NCTU_Formosa 2016.

We changed the general linker to a GS linker to optimize the function of Sf1a.


Previous Part:(BBa_K1974022)

The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 (BBa_I712074), strong ribosome binding site (BBa_B0034), Sf1a, AAA linker, snowdrop lectin (BBa_K1974020) and the 6X His-Tag (BBa_K1223006).


Figure 1. Previous part


Improvement part

The GS linker we used in this improvement part was also provided by NCTU_Formosa 2016. It contained 18 amino acid sequence, which is a Gly-Gly-Ser repeated linker.

Figure 1. Improvement part


Introduction



Figure 1. Comparison of plant protecting effects


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

1. Elaine Fitches, Martin G. Edwards, Christopher Mee, Eugene Grishin, Angharad M. R. Gatehouse, John P. Edwards, John A. Gatehouse “Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion,” Journal of Insect Physiology, 2004, 50, pp.61-71
2. Elaine C. Fitches, Prashant Pyati, Glenn F. King, John A. Gatehouse, “ Fusion to Snowdrop Lectin Magnifies the Oral Activity of Insecticidal Omega-Hexatoxin-Hv1a Peptide by Enabling Its Delivery to the Central Nervous System,”
3. Monique J. Windley, Volker Herzig, Slawomir A. Dziemborowicz, Margaret C. Hardy, Glenn F. King and Graham M. Nicholson, “Spider-Venom Peptide as Bioinsecticide,” Toxins Review, 2012, 4, pp. 191-227.
4. A. Lipkin, S. Kozlov, E. Nosyreva, A. Blake, J.D. Windass, E. Grishin (2001, April 9). Novel insecticidal toxins from the venom of the spider Segestria florentina. Toxicon, 40, 125-130.