Difference between revisions of "Part:BBa K2680281:Design"

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===Source===
 
===Source===
  
−
"In enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of the type II bacterial quorum sensing molecule (AI-2). This phosphorylation is required for AI-2 sequestration in the cytoplasm and subsequent derepression of AI-2-related genes necessary for quorum development." <sup>1</sup>
+
"In enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of the type II bacterial quorum sensing molecule (AI-2). This phosphorylation is required for AI-2 sequestration in the cytoplasm and subsequent derepression of AI-2-related genes necessary for quorum development."<sup>1</sup>
  
 
===References===
 
===References===
 
Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516
 
Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516

Revision as of 16:25, 15 October 2018


3G LsRK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1647


Design Notes

Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"In enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of the type II bacterial quorum sensing molecule (AI-2). This phosphorylation is required for AI-2 sequestration in the cytoplasm and subsequent derepression of AI-2-related genes necessary for quorum development."1

References

Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516