Difference between revisions of "Part:BBa K2680281:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
To do
 
To do
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+
Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
  
  

Revision as of 16:19, 15 October 2018


3G LsRK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1647


Design Notes

To do Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.


Source

To do

References