Difference between revisions of "Part:BBa K2834002"

Latest revision as of 16:16, 15 October 2018

6x His-Tag

Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).

Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006

References

Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2834002 short</partinfo>
+<p align="justify">
+Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).</p><br>
+<p>
-Defensin 2 has selective activity against Gram-positive bacteria. This peptide disrupts the permeability barrier of the cytoplasmic membrane by forming channels, resulting in a loss of cytoplasmic K+, a partial depolarization of the inner membrane, a decrease in cytoplasmic ATP, and inhibition of respiration processes.
+Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006
-Cociancich, S., Ghazi, A., Hetru, C., Hoffman, J. A., & Letellier, L. (1993). Insect Defensin, an Inducible Antibacterial Peptide, Forms Voltage-dependent Channels in Micrococcus luteus. The Journal of Biological Chemistry, 268(26), 19239-19245
+===References===
+Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.
+</p><br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===