Difference between revisions of "Part:BBa K2599016"

Revision as of 15:57, 15 October 2018

T7 Promoter+RBS+Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag

NCTU_Formosa 2018 designed this sequence to improve the part from NCTU_Formosa 2016.

We changed the general linker to a GS linker to optimize the function of Sf1a.

The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 (BBa_I712074), strong ribosome binding site (BBa_B0034), Sf1a, linker, snowdrop lectin (BBa_K1974020) and the 6X His-Tag (BBa_K1223006).

Figure 1. Improvement part

Introduction

Figure 1. Comparison of plant protecting effects

Sequence and Features

Assembly Compatibility:

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 The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], strong ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], Sf1a, linker, snowdrop lectin [https://parts.igem.org/Part:BBa_K1974020 (BBa_K1974020)] and the 6X His-Tag [https://parts.igem.org/Part:BBa_K1223006 (BBa_K1223006)].
+{{#tag:html|<img style="width: 60%; padding-left: 18%;" src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" alt="" />}}
+<div style="width:40%; padding-left: 28%;"><p style="padding-top: 12px; font-size: 10px; text-align: center;"><b>Figure 1.</b> Improvement part</p></div>