Difference between revisions of "Part:BBa K2599016"

Revision as of 15:06, 15 October 2018

T7 Promoter+RBS+Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag

NCTU_Formosa 2018 designed this sequence to improve the part from NCTU_Formosa 2016. We changed the general linker to a GS linker to optimize the function of Sf1a.

Previous Part: [1]

The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 (BBa_I712074), strong ribosome binding site (BBa_B0034), Sf1a, linker, snowdrop lectin (BBa_K1974020) and the 6X His-Tag (BBa_K1223006).

Introduction

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:05, 15 October 2018 (view source) Yen-ling (Talk \| contribs) ← Older edit		Revision as of 15:06, 15 October 2018 (view source) Yen-ling (Talk \| contribs) Newer edit →
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−	Previous Part: BBa_K1974022	+	===Previous Part: [https://parts.igem.org/Part:BBa_K1974022(BBa_K1974022)]===

	The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], strong ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], Sf1a, linker, snowdrop lectin [https://parts.igem.org/Part:BBa_K1974020 (BBa_K1974020)] and the 6X His-Tag [https://parts.igem.org/Part:BBa_K1223006 (BBa_K1223006)].		The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], strong ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], Sf1a, linker, snowdrop lectin [https://parts.igem.org/Part:BBa_K1974020 (BBa_K1974020)] and the 6X His-Tag [https://parts.igem.org/Part:BBa_K1223006 (BBa_K1223006)].