Difference between revisions of "Part:BBa K2680119"
 
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<sup>1</sup>Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051
 
<sup>1</sup>Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051
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Elowitz repressilator paper: Elowitz, M. B., & Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 403(6767), 335-338. doi:10.1038/35002125
  
  

Latest revision as of 14:45, 15 October 2018


3G CI-repressible promoter

CI-repressible promoter BBa_R0051 flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

"The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein). [...] Intrinsic noise value: 0.0869 (compare with R0010: 0.0707; R0011: 0.0040). See [http://2015.igem.org/Team:William_and_Mary William_and_Mary iGEM 2015]."1

Usage and Biology

"Strong promoter."1

References

1Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051

Elowitz repressilator paper: Elowitz, M. B., & Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 403(6767), 335-338. doi:10.1038/35002125


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 106