Difference between revisions of "Part:BBa K2541404:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sfGFP(BbsI free) is BbsI restriction site free and can be used in Golden Gate assembly. | |
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===Source=== | ===Source=== | ||
| − | + | sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from 5’-gaagac-3’ to 5’-gaggat-3’. And other sequences are derived from Registry of Standard Biological Parts. | |
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===References=== | ===References=== | ||
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[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88. | [3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88. | ||
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| + | [4]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490. | ||
Latest revision as of 14:34, 15 October 2018
sfGFP(BbsI free) measurement device
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 74
Design Notes
The sfGFP(BbsI free) is BbsI restriction site free and can be used in Golden Gate assembly.
Source
sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from 5’-gaagac-3’ to 5’-gaggat-3’. And other sequences are derived from Registry of Standard Biological Parts.
References
[1]Southward CM, Surette MG. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191–1196.
[2]Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38.
[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.
[4]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490.