Difference between revisions of "Part:BBa K2632001"
 
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<partinfo>BBa_K2381000 short</partinfo>
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<partinfo>BBa_K2632001 short</partinfo>
  
 
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pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is FAD which is normal in nearly all organisms.
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Gene ent located in the cluster of enterobactin (ent) genes. In respond to iron deprivation, <i>Salmonella</i> <i>enterica</i> serovar Typhimurim secretes siderophores and enterobactin in SCV condition to sense and adapt to the intracellular environment of different types of host cells.  
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This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag
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<h1>Notice</h1>
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<a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381001</a>.It's function is proved by parts                   
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<p1>
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<a href="https://parts.igem.org/Part:BBa_K2381017">BBa_K2381017</a>
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This part carried it's native RBS. You can attach a CDS to it. <br>This part should be in a low copy plasmid  such as PSB4K5, or it will express before <i>Salmonella</i>'s entry to cell. </br>
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<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.
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<br> Use in <i>Salmonella</i> only.</br>
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</p1>
  
  
 
<h3>Reference</h3>
 
<h3>Reference</h3>
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<p>Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256</p>
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<p>
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1. Crouch, M. L. V, Castor, M., Karlinsey, J. E., Kalhorn, T. & Fang, F. C. Biosynthesis and IroC-dependent export of the siderophore salmochelin are essential for virulence of <i>Salmonella</i> <i>enterica</i> serovar Typhimurium. Mol. Microbiol. 67, 971–983 (2008).  
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2.Liu, J., Walsh, C. T., Liu, J., Quinn, N. & Berchtold, G. A. Overexpression, Purification, and Characterization of Isochorismate Synthase (EntC), the First Enzyme Involved in the Biosynthesis of Enterobactin from Chorismate. Biochemistry29, 1417–1425 (1990).                     
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3. Hautefort, I. et al. During infection of epithelial cells <i>Salmonella</i> <i>enterica</i> serovar Typhimurium undergoes a time-dependent transcriptional adaptation that results in simultaneous expression of three type 3 secretion systems. Cell. Microbiol. 10, 958–984 (2008).</p>
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2632003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2632001 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2381000 parameters</partinfo>
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<partinfo>BBa_K2632001 parameters</partinfo>
 
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Latest revision as of 13:37, 15 October 2018

Promoter ent Actived Intracelluraly in Salmonella

Gene ent located in the cluster of enterobactin (ent) genes. In respond to iron deprivation, Salmonella enterica serovar Typhimurim secretes siderophores and enterobactin in SCV condition to sense and adapt to the intracellular environment of different types of host cells.

Notice

This part carried it's native RBS. You can attach a CDS to it.
This part should be in a low copy plasmid such as PSB4K5, or it will express before Salmonella's entry to cell.

Use in Salmonella only.

Reference

1. Crouch, M. L. V, Castor, M., Karlinsey, J. E., Kalhorn, T. & Fang, F. C. Biosynthesis and IroC-dependent export of the siderophore salmochelin are essential for virulence of Salmonella enterica serovar Typhimurium. Mol. Microbiol. 67, 971–983 (2008). 2.Liu, J., Walsh, C. T., Liu, J., Quinn, N. & Berchtold, G. A. Overexpression, Purification, and Characterization of Isochorismate Synthase (EntC), the First Enzyme Involved in the Biosynthesis of Enterobactin from Chorismate. Biochemistry29, 1417–1425 (1990). 3. Hautefort, I. et al. During infection of epithelial cells Salmonella enterica serovar Typhimurium undergoes a time-dependent transcriptional adaptation that results in simultaneous expression of three type 3 secretion systems. Cell. Microbiol. 10, 958–984 (2008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]