Difference between revisions of "Part:BBa K2666008"
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=== I. Part BBa K2666001 : Function === | === I. Part BBa K2666001 : Function === | ||
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=== II. Proof of function === | === II. Proof of function === | ||
| − | [[File:T--Montpellier--R0-RFP Cytometer mtp.PNG| | + | [[File:T--Montpellier--R0-RFP Cytometer mtp.PNG|350px]] |
| − | '''Figure 2''' : | + | '''Figure 2''' : Quantity of E.coli that produce RFP by low cytometer. In gray, the control without plasmid. In pink, the E. coli population with the construction R0-RFP. |
[[File:T--Montpellier--R0-RFP cytometer2 mtp.PNG|500px]] | [[File:T--Montpellier--R0-RFP cytometer2 mtp.PNG|500px]] | ||
| − | '''Figure 3''' : | + | '''Figure 3''' : Number of E.coli that produce RFP by low cytometer. A) E. coli population with the construction R0-RFP B) Control without plasmid. |
=== III. Design Considerations === | === III. Design Considerations === | ||
Latest revision as of 08:15, 15 October 2018
Contents
RFP with B. subtilis specific strong promoter R0
I. Part BBa K2666001 : Function
The Montpellier iGEM team 2018 designed a construction that produce RFP in Bacillus subtilis (http://2018.igem.org/Team:Montpellier/Toolbox). For that, we used the promoter R0, a B.subtilis specific strong promoter [1]. Figure 1 illustrates the detailed design.
Figure 1 : Construct design. The sequence that encode RFP fusionned with a strong promoter R0.
The objective is to check the strength of several construction in E.coli, L.jensenii & B.subtilis.
II. Proof of function
Figure 2 : Quantity of E.coli that produce RFP by low cytometer. In gray, the control without plasmid. In pink, the E. coli population with the construction R0-RFP.
Figure 3 : Number of E.coli that produce RFP by low cytometer. A) E. coli population with the construction R0-RFP B) Control without plasmid.
III. Design Considerations
We added spacers to all of our constructions to unable easier use of the sequence and separation of the different genes of the sequences. We used two Terminators to our sequences :BBa_B0014 & BBa_B0015 to ensure the stopping of the transcription.
Reference:
[1] Guiziou, Sarah, et al. "A part toolbox to tune genetic expression in Bacillus subtilis." Nucleic acids research 44.15 (2016): 7495-7508.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 62
Illegal BamHI site found at 194 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]