Difference between revisions of "Part:BBa K2559004"

Revision as of 07:02, 15 October 2018

E coli promoter of sgRNA(PdapA) The BBa_K2559004 , dapA promoter is an endogenous promoter in Escherichia coli K 12

Usage and Biology

We have obtained this promoter from a database, PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes documentation on the location of experimentally identified mRNA transcriptional start sites on the E. coli chromosome, as well as the actual sequences in the promoter region. The database is currently updated as of July 2000 and includes 471 entries. We have built up a model to measure the expression strength of these promoters and tested the prplJ, pdapA, and pcaiF expression strength by comparing the fluorescent intensity.You can use PromEC and our model to obtain more useful promoter information. The eGFP expression in E.coli(DH5 alpha) and was driven by prplj/pdapa/pcaif promoter.

Figure1 The result of the fluorescent intensity measurement

Figure2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.

The part is facilitate the in-depth research for other teams!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
+We have obtained this promoter from a database, PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes documentation on the location of experimentally identified mRNA transcriptional start sites on the E. coli chromosome, as well as the actual sequences in the promoter region. The database is currently updated as of July 2000 and includes 471 entries.
+We have built up a model to measure the expression strength  of these promoters and tested the prplJ, pdapA, and pcaiF expression strength by comparing the fluorescent intensity.You can use PromEC  and our model to obtain more useful promoter information.
+The eGFP expression in E.coli(DH5 alpha) and was driven by prplj/pdapa/pcaif promoter.
+[[File:Scau-china-2018-11.png|800px|thumb|center|Figure1 The result of the fluorescent intensity measurement ]]
+[[File:Scau-china-2018-12.png|800px|thumb|center|Figure2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.]]
+The part is facilitate the in-depth research for other teams!
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