Difference between revisions of "Part:BBa K2680122"
 
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<partinfo>BBa_K2680122 short</partinfo>
 
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pLsrA promoter in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
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[https://parts.igem.org/Part:BBa_K2680527 pLsrA promoter] flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
  
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Flanked by prefix sticky end A and suffix sticky end B.
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LsrA is one of four genes in the lsr quorum-sensing operon that aids in the uptake of Autoinducer-2 (AI-2) into the cell. Specifically, "lsrA encodes the ATPase that provides energy for AI-2 transport." Phosphorylated AI-2 (phospho-AI-2) induces the lsr operon by binding to LsrR, which represses the operon.<sup>1</sup>
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William and Mary iGEM encountered the promoter for lsrA through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki].
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===References===
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<sup>1</sup>Xavier, K. B., & Bassler, B. L. (2005). Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. Journal of Bacteriology, 187(1), 238–248. http://doi.org/10.1128/JB.187.1.238-248.2005
  
 
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Latest revision as of 01:35, 15 October 2018


3G pLsrA

pLsrA promoter flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

LsrA is one of four genes in the lsr quorum-sensing operon that aids in the uptake of Autoinducer-2 (AI-2) into the cell. Specifically, "lsrA encodes the ATPase that provides energy for AI-2 transport." Phosphorylated AI-2 (phospho-AI-2) induces the lsr operon by binding to LsrR, which represses the operon.1

William and Mary iGEM encountered the promoter for lsrA through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki].

References

1Xavier, K. B., & Bassler, B. L. (2005). Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. Journal of Bacteriology, 187(1), 238–248. http://doi.org/10.1128/JB.187.1.238-248.2005

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 154