Difference between revisions of "Part:BBa K2739007"
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This composite part is improved from the composite part of BBa_K390501, LacP-RBS-PhaP-HlyA-Terminator, which the Phasin part sequencing result revealed a stop codon. This improved composite part has the stop condon removed. | This composite part is improved from the composite part of BBa_K390501, LacP-RBS-PhaP-HlyA-Terminator, which the Phasin part sequencing result revealed a stop codon. This improved composite part has the stop condon removed. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | https://static.igem.org/mediawiki/parts/1/17/T--Edinburgh_OG_BBa_K2739007--image_1.jpeg | ||
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| + | Figure 1. Agarose gel electrophoresis of Lac promoter-Phasin-HlyA product. A. PCR product of Lac promoter-Phasin-HlyA with stop codon removal. B. Enzyme digestion of Lac promoter-Phasin-HlyA PCR product for stop codon removal (lane 1: 1 kb DNA ladder; 2: EcoR1 and HindIII double digestion) with the label of HlyA + pSB1C3 Backbone and Phasin. | ||
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Revision as of 23:31, 14 October 2018
LacP-RBS-PhaP-HlyA-Terminator
This composite part is improved from the composite part of BBa_K390501, LacP-RBS-PhaP-HlyA-Terminator, which the Phasin part sequencing result revealed a stop codon. This improved composite part has the stop condon removed.
Usage and Biology
Figure 1. Agarose gel electrophoresis of Lac promoter-Phasin-HlyA product. A. PCR product of Lac promoter-Phasin-HlyA with stop codon removal. B. Enzyme digestion of Lac promoter-Phasin-HlyA PCR product for stop codon removal (lane 1: 1 kb DNA ladder; 2: EcoR1 and HindIII double digestion) with the label of HlyA + pSB1C3 Backbone and Phasin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]