Latest revision as of 18:10, 27 June 2008

ESSENTIAL ITEMS FOR ALL PARTS

Main Page

(10 points) (+1)

Answers the question: "What is it?"

0. Designer/iGEM team.* (1)

1. Part number. (alternatively “Accession number”)* (0)

2. Short name.* (1)

3. Extended name. (alternatively an informative title)* (2)

4. Origin (biological species if available, otherwise name the source: "supplied by __________" or "designed, based on ____________").* (3)

5. A short abstract that tells (a) what the part does (function), (b) how it works (mechanism) and (c) why you made it (purpose).* (3)

6. Dependencies: other parts, special materials, cofactors, chassis, etc. (1) (if applicable)

Design Page

(10 points) (+5)

Answers the question: "How did you make it?"

7. Raison d’être for constructing the part.* (3) Links to related parts from the same project. (2) [Details in addition to those under items (5) and (6).]

8. DNA sequence without the BioBrick ends.* (0)

9. Primers used (if part was created using PCR).* (2)

10. Synthesis details (if part was created by DNA synthesis*). (1)

11. Special design features: (a) insertion/removal of BioBrick cloning sites, (b) codon optimization, (c) reverse orientation, (d) added tags…* (2)

13. Graphical sequence bar with all appropriate feature annotations. (1)

14. One-to-three references to literature* on the part’s biological function and uses in synthetic biology. Frequently there is a key reference reporting on the first use or design of a modified version of the protein (see entries under “Reporters”). If no published references are available, cite other sources used (such as online, open-access textbooks, Open Wetware pages, etc.) (2)

15. Difficulties/pitfalls encountered in the design process. Unworkable designs, necessary revisions etc. If everything worked exactly as planned, say so!* (1)

16.? List all applicable patents and materials transfer agreements (MTAs). [Give link to disclaimer page on IP & iGEM issues.] (1)

Experience Page

(3 points) (+4)

Answers the question: "How well does it work?"

17. Summary of actual applications to date in originating laboratory.* (3)

18. Measurements or tests of part. Include efficiency. (2)

19. Uploaded data, figures etc. can be put on the Registry wiki and linked here. (2)

Hard Information Page

(2 points) (+1)

Gives reference data.

20. Sequence (FASTA format).* (0)

21. Exact source of the original sequence(s) used to construct the part. GenBank accession number (“VERSION”) and/or “GI” (aka “gi”) number if available. Otherwise any available published sequence or other source of information, including experimental sequence reads (which can be put on the Registry wiki and linked here).* (2)

22. More features (e.g., exact details of modified sequences). (1)

POSSIBLE ENHANCEMENTS

Suggestions about how to liven it up...(use visual materials!)

Main Page

(+4)

1. Structure cartoon(s). PDB_IDs. (2)

2. Figures relating to the function and/or physical characteristics of the part. (Include forward and reverse efficiencies if known.) (2)

Experience Page

(+5)

3. Tables of measurements. (3)

4. Figures depicting functional output. (2)

5. Links to additional databases. (2)