Difference between revisions of "Part:BBa K2680124"

Latest revision as of 22:10, 14 October 2018

3G pTlpAr

pTlpAr flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pTlpAr is the reverse complement of pTlpA, a promoter repressed by heat-sensitive protein TlpA. pTlpAr transcribes 200-fold weaker than pTlpA.¹ Flanked by prefix sticky end A and suffix sticky end B.

References

¹Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 41
Illegal BsaI.rc site found at 109

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2680124 short</partinfo>
-G pTlpAr
+pTlpAr flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
+pTlpAr is the reverse complement of [https://parts.igem.org/Part:BBa_K2500003 pTlpA], a promoter repressed by heat-sensitive protein TlpA. pTlpAr transcribes 200-fold weaker than pTlpA.<sup>1</sup> Flanked by prefix sticky end A and suffix sticky end B.
+===References===
+<sup>1</sup>Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233
 <!-- Add more about the biology of this part here