Difference between revisions of "Part:BBa K2680123"

Latest revision as of 21:45, 14 October 2018

3G pTlpA

pTlpA promoter BBa_K2500003 flanked by prefix sticky end A and suffix sticky end B in in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI^Q) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."¹ See pTlpAr BBa_K2680124 for the reverse complement of pTlpA (also in 3G compatible form).

References

¹Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 41
Illegal BsaI.rc site found at 109

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2680123 short</partinfo>
-pTlpA promoter <partinfo>BBa_K2500003</partinfo> in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
+pTlpA promoter <partinfo>BBa_K2500003</partinfo> flanked by prefix sticky end A and suffix sticky end B in in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
-pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI<sup>Q</sup>) driven by the transcription factor &sigma;. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."<sup>1</sup> See pTlpAr <partinfo>BBa_K2680124</partinfo> for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.
+pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI<sup>Q</sup>) driven by the transcription factor &sigma;. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."<sup>1</sup> See pTlpAr <partinfo>BBa_K2680124</partinfo> for the reverse complement of pTlpA (also in 3G compatible form).
 ===References===
-<sup>1</sup>Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., &amp; Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. <I>Nature Chemical Biology</I> doi:10.1038/nchembio.2233, 2 (2016).
+<sup>1</sup>Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233
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