Difference between revisions of "Part:BBa K2660008"

Revision as of 13:12, 14 October 2018

nMag - Light-activated protein

This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The negative Magnet (nMag) when irradiated by a blue light change through electrostatic interactions.

This part can be used with pMag (BBa_K2660009) to join different domains of a protein of interest when irradiated by blue light

References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 87
1000
COMPATIBLE WITH RFC[1000]

Revision as of 13:12, 14 October 2018 (view source) VictorNJ (Talk \| contribs) ← Older edit		Revision as of 13:12, 14 October 2018 (view source) VictorNJ (Talk \| contribs) Newer edit →
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	- Light-activated protein		- Light-activated protein

−	This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The <b>negative Magnet (nMag)<b> when irradiated by a blue light change through electrostatic interactions.	+	This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The <b>negative Magnet (nMag)</b> when irradiated by a blue light change through electrostatic interactions.