Difference between revisions of "Part:BBa K2871000"
Nleelahakorn (Talk | contribs) |
Nleelahakorn (Talk | contribs) |
||
| Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Part Characterization | ||
| + | |||
| + | The Map20 T3SS signal | ||
<!-- --> | <!-- --> | ||
Revision as of 11:36, 14 October 2018
Map20, T3SS export signal peptide from Map gene
Map20 is a signal sequence encoding a 20 amino acid long signal peptide derived from the map gene from the Enteropathogenic E. coli strain E22. It is used as an N-terminal tag for a protein to be translocated by the E. coli type-3-secretion system (T3SS). This T3SS signal tag is described by Charpentier & Oswald (2004) to be sufficient to target proteins to E. coli type III secretion pathway. Its short length seemed to be attractive to us because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 85
- 1000COMPATIBLE WITH RFC[1000]