Difference between revisions of "Part:BBa K2637036"

Revision as of 05:20, 14 October 2018

Gal1 promoter+mCherry+ADH1 terminator

We constructed this pRS416 plasmid carrying the genes of mCherry with the Gal1 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2637036 short</partinfo>
-We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.
+We constructed this pRS416 plasmid carrying the genes of mCherry with the Gal1 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.
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