Difference between revisions of "Part:BBa K2637041"

 
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<partinfo>BBa_K2637041 short</partinfo>
 
<partinfo>BBa_K2637041 short</partinfo>
  
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We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. And only if the reporter genes function normally can we ensure that the system we reconstructed in the Saccharomyces cerevisiae succeeds.
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We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process will be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:04, 14 October 2018


Gal2 promoter+NanoLuc+ADH1 terminator

We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process will be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 529