Difference between revisions of "Part:BBa K2680113"
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<partinfo>BBa_K2680113 short</partinfo> | <partinfo>BBa_K2680113 short</partinfo> | ||
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− | <partinfo>BBa_J23115</partinfo> in 3G compatible | + | <partinfo>BBa_J23115</partinfo> flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. |
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+ | J23115 is a weak (0.15x strength of J23100) constitutive promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 21:54, 13 October 2018
3G J23115
BBa_J23115 flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
J23115 is a weak (0.15x strength of J23100) constitutive promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
Illegal NheI site found at 87 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 47
Illegal BsaI.rc site found at 98