Difference between revisions of "Part:BBa K2680123"

Revision as of 21:29, 13 October 2018

3G pTlpA

pTlpA promoter BBa_K2500003 in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G-parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI^Q) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."¹ See pTlpAr BBa_K2680124 for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.

References

¹Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., & Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology doi:10.1038/nchembio.2233, 2 (2016).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 41
Illegal BsaI.rc site found at 109

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2680123 short</partinfo>
-pTlpA promoter <partinfo>BBa_K2500003</partinfo> in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G-parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page] Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI<sup>Q</sup>) driven by the transcription factor &sigma;. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."<sup>1</sup> See pTlpAr <partinfo>BBa_K2680124</partinfo> for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.
+pTlpA promoter <partinfo>BBa_K2500003</partinfo> in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G-parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI<sup>Q</sup>) driven by the transcription factor &sigma;. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."<sup>1</sup> See pTlpAr <partinfo>BBa_K2680124</partinfo> for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.
 ===References===