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Revision as of 21:25, 13 October 2018
__NOTOC__
BBa_K2680123 short
pTlpA promoter BBa_K2500003 in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G-parts, see the William and Mary 3G Parts Page. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacIQ) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."1 See pTlpAr BBa_K2680124 for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.
===References===
1Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., & Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology doi:10.1038/nchembio.2233, 2 (2016).
Sequence and Features
BBa_K2680123 SequenceAndFeatures
