Difference between revisions of "Part:BBa K2680123"
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<partinfo>BBa_K2680123 short</partinfo>
 
<partinfo>BBa_K2680123 short</partinfo>
  
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3G-compatible pTlpA promoter <partinfo>BBa_K2500003</partinfo>. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. pTlpA also transcribes 500-fold weaker in the reverse direction; see pTlpAr <partinfo>BBa_K2680124</partinfo>. Flanked by prefix sticky end A and suffix sticky end B.
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pTlpA promoter <partinfo>BBa_K2500003</partinfo> in a 3G-compatible form. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI<sup>Q</sup>) driven by the transcription factor &sigma;. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."<sup>1</sup> See pTlpAr <partinfo>BBa_K2680124</partinfo> for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.
  
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===References===
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<sup>1</sup>Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., &amp; Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. <I>Nature Chemical Biology</I> <b>13</b>, 76 (2016).
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:04, 13 October 2018


3G pTlpA

pTlpA promoter BBa_K2500003 in a 3G-compatible form. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacIQ) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."1 See pTlpAr BBa_K2680124 for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.

References

1Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., & Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology 13, 76 (2016). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109