Difference between revisions of "Part:BBa K2675097:Design"

 
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===Design Notes===
 
===Design Notes===
−
selection from a random sequence library
+
pAimX(full) promoter of phage phi3T ([[Part:BBa_K2675020|BBa_K2675020]]) with mutations in the putative terminator. These mutations disrupted also the pAimX(2) and pAimX(3) putative promoters.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
selection from a random sequence library
+
selection from a random sequence library derived by PCR with degenerate primers on [[Part:BBa_K2675020|BBa_K2675020]]
  
 
===References===
 
===References===
 +
[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.

Revision as of 20:50, 13 October 2018


pAimX(full-noTerminator-v8) promoter of phage phi3T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 71


Design Notes

pAimX(full) promoter of phage phi3T (BBa_K2675020) with mutations in the putative terminator. These mutations disrupted also the pAimX(2) and pAimX(3) putative promoters.

Source

selection from a random sequence library derived by PCR with degenerate primers on BBa_K2675020

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.