Difference between revisions of "Part:BBa K2668010:Design"

 
 
(2 intermediate revisions by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be purified, this part was cloned in double digested pET28 (NcoI / HindIII) for being expressed with a His Tag only if the unnatural amino acid incorporation occured.
+
<html>
−
 
+
The part BBa_K2668010 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">iGEM Team Toulouse-INSA-UPS Cerberus Project</a>. It was originally designed to bind a variety of compounds to cellulose thanks to its two linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.
 +
</html>
  
  
 
===Source===
 
===Source===
  
−
The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible . The streptavidin part comes from Streptomyces avidinii.
+
The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible. The monomeric streptavidin is the Part:BBa_K1896000.
  
  
  
 
===References===
 
===References===

Latest revision as of 14:00, 13 October 2018


Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668010 comes from the iGEM Team Toulouse-INSA-UPS Cerberus Project. It was originally designed to bind a variety of compounds to cellulose thanks to its two linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.


Source

The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible. The monomeric streptavidin is the Part:BBa_K1896000.


References