Difference between revisions of "Part:BBa K2637022:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Gal4 DNA binding domain fused with CikA gene. CikA has been optimized in yeast. | |
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===Source=== | ===Source=== | ||
| − | + | Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942. | |
===References=== | ===References=== | ||
Latest revision as of 13:55, 13 October 2018
BD-CikA, Gal4 DNA binding domain fused with KaiB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 590
Illegal BamHI site found at 2650
Illegal XhoI site found at 234 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 153
Design Notes
Gal4 DNA binding domain fused with CikA gene. CikA has been optimized in yeast.
Source
Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.