Difference between revisions of "Part:BBa K2669003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <partinfo>BBa_K2669003 Usage and Biology</partinfo> | ||
AmilCP is a blue chromoprotein that originates from the coral Acropora millepora, which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture. The chromoprotein can be used as a quantitative reporter. By performing codon optimization on original native AmilCP, the team of Uppsala 2018 have improved the translation efficiency and prooved the optimized part to have a more stable expression in E.coli trough several generations of growth. | AmilCP is a blue chromoprotein that originates from the coral Acropora millepora, which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture. The chromoprotein can be used as a quantitative reporter. By performing codon optimization on original native AmilCP, the team of Uppsala 2018 have improved the translation efficiency and prooved the optimized part to have a more stable expression in E.coli trough several generations of growth. | ||
Revision as of 13:00, 13 October 2018
Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
Blue chromoprotein Biobrick part containing codon optimized original native AmilCP Part:BBa_K2669002, RBS Part:BBa_J34801, constitutive promotor Part:BBa_J23119 and a double terminator Part:BBa_B0014.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]